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Image Search Results
Journal: JCI Insight
Article Title: Affinity-tuned mesothelin CAR T cells demonstrate enhanced targeting specificity and reduced off-tumor toxicity
doi: 10.1172/jci.insight.186268
Figure Lengend Snippet: ( A ) Schematic representation of the lentiviral vectors containing MSLN CAR variants. ( B ) Relative binding of MSLN CAR variants was estimated by staining CAR-expressing Jurkat T cells with 25 nM of Alexa Fluor 647–conjugated monomeric human and mouse MSLN, using NT cells as control. ( C ) Determination of the equilibrium binding affinities of CAR variants using a saturation binding assay with Alexa Fluor 647–conjugated human and mouse MSLN ( n = 1).
Article Snippet: Two alpacas (obtained from local alpaca farms by Tufts University) were immunized with 5 subcutaneous injections of the C-terminal domains of both recombinant human MSLN (aa 296–580, R&D Systems, Bio-Techne, catalog 3265-MS) and
Techniques: Binding Assay, Staining, Expressing, Control, Saturation Assay